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lipofectamine ltx with plustm reagent transfection reagent  (Thermo Fisher)


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    Structured Review

    Thermo Fisher lipofectamine ltx with plustm reagent transfection reagent
    Lipofectamine Ltx With Plustm Reagent Transfection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lipofectamine+plustm+reagent/lipofectamine+ltx+with+plustm+reagent+transfection+reagent/pmc12147355-44-45-56
    Average 90 stars, based on 1 article reviews
    lipofectamine ltx with plustm reagent transfection reagent - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: R-PTP-κ Inhibits Contact-Dependent Cell Growth by Suppressing E2F Activity.
    Article Snippet: Chang Liver, HeLa, and normal human fibroblast (HFT) cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (Hyclone, Logan, UT, USA). .. For reporter assays, cells were seeded at a density of 2 × 105 cells/well in 6-well plates, incubated for 24 h, and then transfected with 1 μg DNA/well using Lipofectamine PlusTM reagent (Invitrogen, Waltham, MA, USA), according to the supplier’s protocol. ..

    Transfection:

    Article Title: R-PTP-κ Inhibits Contact-Dependent Cell Growth by Suppressing E2F Activity.
    Article Snippet: Chang Liver, HeLa, and normal human fibroblast (HFT) cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (Hyclone, Logan, UT, USA). .. For reporter assays, cells were seeded at a density of 2 × 105 cells/well in 6-well plates, incubated for 24 h, and then transfected with 1 μg DNA/well using Lipofectamine PlusTM reagent (Invitrogen, Waltham, MA, USA), according to the supplier’s protocol. ..

    Article Title: The effect of CPNE7 on periodontal regeneration.
    Article Snippet: Introduction: Preameloblast-conditioned medium (PA-CM), as a mixture of dental epitheliumAc ce pte d M an us cri pt 2 derived factors has been reported to regenerate dentin and periodontal tissues in vitro and in vivo.. Recently, protein copine7 (Cpne7) which was found in PA-CM was suggested to have a beneficial role in periodontal tissue healing.. The aim of this study was to investigate the biological effect of Cpne7 on the proliferation, migration, and cementoblast differentiation of periodontal cells in vitro, and on the regeneration of periodontal tissue using periodontal defect model with canine in vivo.

    Article Title: Hypoxia induces inflammatory microenvironment by priming specific macrophage polarization and modifies LSC behaviour via VEGF-HIF1α signalling
    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) The levels of cytokines, including IL-1β, TNF-α, VEGF, and CCL17 in supernatant were measured with ELISA kits (Nanjing Jingcheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s instructions. .. RNA knockdown siRNA targeting survivin mRNA was followed as: 5′-CACCGCAUCUCUCUACAUUCATT-3′ (sense) and 5′-UGAAUGUAGAGAGAUGCGGUGTT-3′ (antisense); 2 μg survivin siRNA or control siRNA were transiently transfected into cells for 48 h using the Lipofectamine PlusTM reagent (Gibco BRL, USA) following the manufacturer’s instructions and used for further study. .. Apoptosis assay Apoptosis was evaluated using Annexin V-FITC/PI apoptosis detection kit (BD Biosciences, Franklin Lakes, NJ, USA) by flow cytometry analysis.

    Cell Culture:

    Article Title: Downregulation of miR-760 Causes Human Intervertebral Disc Degeneration by Targeting the MyD88/Nuclear Factor-Kappa B Signaling Pathway
    Article Snippet: All constructs were confirmed by sequencing (Cosmo Genetech, Seoul, Korea). .. The cultured primary NP cells were co-transfected with wild-type or mutant MyD88 3′-UTR-Luc reporter plasmid and microRNA using the Lipofectamine PLUSTM reagent (Invitrogen). .. Cell lysates were harvested 48 h after transfection, and luciferase activity was assayed using the Dual-Glo luciferase Assay system (Promega, Madison, WI, United States).

    Mutagenesis:

    Article Title: Downregulation of miR-760 Causes Human Intervertebral Disc Degeneration by Targeting the MyD88/Nuclear Factor-Kappa B Signaling Pathway
    Article Snippet: All constructs were confirmed by sequencing (Cosmo Genetech, Seoul, Korea). .. The cultured primary NP cells were co-transfected with wild-type or mutant MyD88 3′-UTR-Luc reporter plasmid and microRNA using the Lipofectamine PLUSTM reagent (Invitrogen). .. Cell lysates were harvested 48 h after transfection, and luciferase activity was assayed using the Dual-Glo luciferase Assay system (Promega, Madison, WI, United States).

    Plasmid Preparation:

    Article Title: Downregulation of miR-760 Causes Human Intervertebral Disc Degeneration by Targeting the MyD88/Nuclear Factor-Kappa B Signaling Pathway
    Article Snippet: All constructs were confirmed by sequencing (Cosmo Genetech, Seoul, Korea). .. The cultured primary NP cells were co-transfected with wild-type or mutant MyD88 3′-UTR-Luc reporter plasmid and microRNA using the Lipofectamine PLUSTM reagent (Invitrogen). .. Cell lysates were harvested 48 h after transfection, and luciferase activity was assayed using the Dual-Glo luciferase Assay system (Promega, Madison, WI, United States).

    Article Title: Reinforced-hydrogel encapsulated hMSCs towards brain injury treatment by trans-septal approach.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Luciferase:

    Article Title: Chondrogenic primed extracellular vesicles activate miR-455/SOX11/FOXO axis for cartilage regeneration and osteoarthritis treatment
    Article Snippet: To construct the SOX11 3′-UTR-Luc reporter plasmid, a wild-type (Wt) or mutant (Mut) fragment of the 3′-UTR of SOX11 containing the predicted miR-455 binding sites was PCR-amplified and inserted into the psi-CHECKTM-2 vector (Promega, Madison, WI). .. To conduct the luciferase assay, human BMSCs were seeded into a 96-well plate and co-transfected with WT- or Mut-SOX11 3′-UTR-Luc reporter plasmids and a miR-455 mimic, inhibitor, or negative control with Lipofectamine PLUSTM reagent (Invitrogen, China). .. At 48 h after transfection, the cell lysates were harvested for luciferase activity determination using the Dual-Glo Luciferase Assay system (Promega, Madison, WI, USA) and were normalized to firefly luciferase activity.

    Article Title: Chondrogenic primed extracellular vesicles activate miR-455/SOX11/FOXO axis for cartilage regeneration and osteoarthritis treatment.
    Article Snippet: To construct the SOX11 3′-UTR-Luc reporter plasmid, a wild-type (Wt) or mutant (Mut) fragment of the 3′-UTR of SOX11 containing the predicted miR-455 binding sites was PCR-amplified and inserted into the psi-CHECKTM-2 vector (Promega, Madison, WI). .. To conduct the luciferase assay, human BMSCs were seeded into a 96-well plate and co-transfected with WT- or Mut-SOX11 3′-UTRLuc reporter plasmids and a miR-455 mimic, inhibitor, or negative control with Lipofectamine PLUSTM reagent (Invitrogen, China). .. At 48 h after transfection, the cell lysates were harvested for luciferase activity determination using the Dual-Glo Luciferase Assay system (Promega, Madison, WI, USA) and were normalized to firefly luciferase activity.

    Negative Control:

    Article Title: Chondrogenic primed extracellular vesicles activate miR-455/SOX11/FOXO axis for cartilage regeneration and osteoarthritis treatment
    Article Snippet: To construct the SOX11 3′-UTR-Luc reporter plasmid, a wild-type (Wt) or mutant (Mut) fragment of the 3′-UTR of SOX11 containing the predicted miR-455 binding sites was PCR-amplified and inserted into the psi-CHECKTM-2 vector (Promega, Madison, WI). .. To conduct the luciferase assay, human BMSCs were seeded into a 96-well plate and co-transfected with WT- or Mut-SOX11 3′-UTR-Luc reporter plasmids and a miR-455 mimic, inhibitor, or negative control with Lipofectamine PLUSTM reagent (Invitrogen, China). .. At 48 h after transfection, the cell lysates were harvested for luciferase activity determination using the Dual-Glo Luciferase Assay system (Promega, Madison, WI, USA) and were normalized to firefly luciferase activity.

    Article Title: Chondrogenic primed extracellular vesicles activate miR-455/SOX11/FOXO axis for cartilage regeneration and osteoarthritis treatment.
    Article Snippet: To construct the SOX11 3′-UTR-Luc reporter plasmid, a wild-type (Wt) or mutant (Mut) fragment of the 3′-UTR of SOX11 containing the predicted miR-455 binding sites was PCR-amplified and inserted into the psi-CHECKTM-2 vector (Promega, Madison, WI). .. To conduct the luciferase assay, human BMSCs were seeded into a 96-well plate and co-transfected with WT- or Mut-SOX11 3′-UTRLuc reporter plasmids and a miR-455 mimic, inhibitor, or negative control with Lipofectamine PLUSTM reagent (Invitrogen, China). .. At 48 h after transfection, the cell lysates were harvested for luciferase activity determination using the Dual-Glo Luciferase Assay system (Promega, Madison, WI, USA) and were normalized to firefly luciferase activity.

    Expressing:

    Article Title: The effect of CPNE7 on periodontal regeneration.
    Article Snippet: Introduction: Preameloblast-conditioned medium (PA-CM), as a mixture of dental epitheliumAc ce pte d M an us cri pt 2 derived factors has been reported to regenerate dentin and periodontal tissues in vitro and in vivo.. Recently, protein copine7 (Cpne7) which was found in PA-CM was suggested to have a beneficial role in periodontal tissue healing.. The aim of this study was to investigate the biological effect of Cpne7 on the proliferation, migration, and cementoblast differentiation of periodontal cells in vitro, and on the regeneration of periodontal tissue using periodontal defect model with canine in vivo.

    Knockdown:

    Article Title: Hypoxia induces inflammatory microenvironment by priming specific macrophage polarization and modifies LSC behaviour via VEGF-HIF1α signalling
    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) The levels of cytokines, including IL-1β, TNF-α, VEGF, and CCL17 in supernatant were measured with ELISA kits (Nanjing Jingcheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s instructions. .. RNA knockdown siRNA targeting survivin mRNA was followed as: 5′-CACCGCAUCUCUCUACAUUCATT-3′ (sense) and 5′-UGAAUGUAGAGAGAUGCGGUGTT-3′ (antisense); 2 μg survivin siRNA or control siRNA were transiently transfected into cells for 48 h using the Lipofectamine PlusTM reagent (Gibco BRL, USA) following the manufacturer’s instructions and used for further study. .. Apoptosis assay Apoptosis was evaluated using Annexin V-FITC/PI apoptosis detection kit (BD Biosciences, Franklin Lakes, NJ, USA) by flow cytometry analysis.

    Control:

    Article Title: Hypoxia induces inflammatory microenvironment by priming specific macrophage polarization and modifies LSC behaviour via VEGF-HIF1α signalling
    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) The levels of cytokines, including IL-1β, TNF-α, VEGF, and CCL17 in supernatant were measured with ELISA kits (Nanjing Jingcheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s instructions. .. RNA knockdown siRNA targeting survivin mRNA was followed as: 5′-CACCGCAUCUCUCUACAUUCATT-3′ (sense) and 5′-UGAAUGUAGAGAGAUGCGGUGTT-3′ (antisense); 2 μg survivin siRNA or control siRNA were transiently transfected into cells for 48 h using the Lipofectamine PlusTM reagent (Gibco BRL, USA) following the manufacturer’s instructions and used for further study. .. Apoptosis assay Apoptosis was evaluated using Annexin V-FITC/PI apoptosis detection kit (BD Biosciences, Franklin Lakes, NJ, USA) by flow cytometry analysis.



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    a, b The mRNA expression level of KLK7 in <t>H4</t> cells treated with 30 µM memantine (Mem) (n=6, Student’s t -test, p=0.003) (a) , 100 µM NMDA, and 100 µM L-glutamate (L-Glu) (n=3, Dunnett’s test, p Ctrl vs NMDA <0.0001, p Ctrl vs L-Glu <0.0001) (b) , respectively. c Schematic representation of Aβ degradation assay in <t>H4</t> <t>cells.</t> Synthetic Aβ40 was added to the conditioned medium of H4 cells treated with memantine. After 24 hours, remaining Aβ40 in conditioned medium was detected by immunoblotting. d The Aβ-degrading activity of H4 cells treated with 30 µM memantine. A representative immunoblot and quantified results are shown (Input=1.0, n=3, Student’s t -test, p=0.0205). e Cell viability of H4 cells treated with 30 µM memantine (n=3, Student’s t -test, p=0.3927). All data are shown as mean±SEM. *p<0.05, **p<0.01, ****p<0.0001; n.s., non-significant.
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    a, b The mRNA expression level of KLK7 in <t>H4</t> cells treated with 30 µM memantine (Mem) (n=6, Student’s t -test, p=0.003) (a) , 100 µM NMDA, and 100 µM L-glutamate (L-Glu) (n=3, Dunnett’s test, p Ctrl vs NMDA <0.0001, p Ctrl vs L-Glu <0.0001) (b) , respectively. c Schematic representation of Aβ degradation assay in <t>H4</t> <t>cells.</t> Synthetic Aβ40 was added to the conditioned medium of H4 cells treated with memantine. After 24 hours, remaining Aβ40 in conditioned medium was detected by immunoblotting. d The Aβ-degrading activity of H4 cells treated with 30 µM memantine. A representative immunoblot and quantified results are shown (Input=1.0, n=3, Student’s t -test, p=0.0205). e Cell viability of H4 cells treated with 30 µM memantine (n=3, Student’s t -test, p=0.3927). All data are shown as mean±SEM. *p<0.05, **p<0.01, ****p<0.0001; n.s., non-significant.
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    Image Search Results


    a, b The mRNA expression level of KLK7 in H4 cells treated with 30 µM memantine (Mem) (n=6, Student’s t -test, p=0.003) (a) , 100 µM NMDA, and 100 µM L-glutamate (L-Glu) (n=3, Dunnett’s test, p Ctrl vs NMDA <0.0001, p Ctrl vs L-Glu <0.0001) (b) , respectively. c Schematic representation of Aβ degradation assay in H4 cells. Synthetic Aβ40 was added to the conditioned medium of H4 cells treated with memantine. After 24 hours, remaining Aβ40 in conditioned medium was detected by immunoblotting. d The Aβ-degrading activity of H4 cells treated with 30 µM memantine. A representative immunoblot and quantified results are shown (Input=1.0, n=3, Student’s t -test, p=0.0205). e Cell viability of H4 cells treated with 30 µM memantine (n=3, Student’s t -test, p=0.3927). All data are shown as mean±SEM. *p<0.05, **p<0.01, ****p<0.0001; n.s., non-significant.

    Journal: bioRxiv

    Article Title: Inhibition of NF-κB signaling pathway in astrocytes facilitates amyloid-β clearance by kallikrein-related peptidase 7

    doi: 10.1101/2025.03.02.641088

    Figure Lengend Snippet: a, b The mRNA expression level of KLK7 in H4 cells treated with 30 µM memantine (Mem) (n=6, Student’s t -test, p=0.003) (a) , 100 µM NMDA, and 100 µM L-glutamate (L-Glu) (n=3, Dunnett’s test, p Ctrl vs NMDA <0.0001, p Ctrl vs L-Glu <0.0001) (b) , respectively. c Schematic representation of Aβ degradation assay in H4 cells. Synthetic Aβ40 was added to the conditioned medium of H4 cells treated with memantine. After 24 hours, remaining Aβ40 in conditioned medium was detected by immunoblotting. d The Aβ-degrading activity of H4 cells treated with 30 µM memantine. A representative immunoblot and quantified results are shown (Input=1.0, n=3, Student’s t -test, p=0.0205). e Cell viability of H4 cells treated with 30 µM memantine (n=3, Student’s t -test, p=0.3927). All data are shown as mean±SEM. *p<0.05, **p<0.01, ****p<0.0001; n.s., non-significant.

    Article Snippet: For transient expression of the luciferase constructs in H4 cells, Lipofectamine ® LTX Reagent & PlusTM Reagent (Thermo Fisher Scientific, catalog #15338100) was used following the manufacturer’s protocol.

    Techniques: Expressing, Degradation Assay, Western Blot, Activity Assay

    a Schematic representation of the establishment of luciferase constructs (Empty and h238 WT) and H4 stable cell lines. b, c Luciferase activity of H4 h238 WT treated with 100 µM NMDA, 100 µM L-glutamate (n=4, Dunnett’s test, p Ctrl vs NMDA =0.0038, p Ctrl vs L-Glu =0.0057) (b) , and 30 µM memantine (n=5, Student’s t -test, p=0.0003) (c) , respectively. d Schematic representation of luciferase constructs containing a deletion mutant and a motif mutant of the κB motif in the 238-bp KLK7 promoter region (h238 ΔκB motif and h238 mut-κB motif). e Luciferase activity of each luciferase construct transiently expressed in H4 cells (n=4, Dunnett’s test, p h238 WT vs h238 ΔκB motif =0.0432, p h238 WT vs h238 mut-κB motif =0.0318). f, g Luciferase activity of H4 h238 ΔκB motif (n=4, Dunnett’s test, p Ctrl vs NMDA =0.4847, p Ctrl vs L-Glu =0.3969) (f) and H4 h238 mut-κB motif (n=4, Dunnett’s test, p Ctrl vs NMDA =0.3716, p Ctrl vs L-Glu =0.5012) (g) treated with 100 µM NMDA or 100 µM L-glutamate. h, i Luciferase activity of H4 h238 ΔκB motif (n=5, Student’s t -test, p=0.8164) (h) and H4 h238 mut-κB motif (n=5, Student’s t -test, p=0.5011) (i) treated with 30 µM memantine. All data are shown as mean±SEM. *p<0.05, **p<0.01, ***p<0.001; n.s., non-significant.

    Journal: bioRxiv

    Article Title: Inhibition of NF-κB signaling pathway in astrocytes facilitates amyloid-β clearance by kallikrein-related peptidase 7

    doi: 10.1101/2025.03.02.641088

    Figure Lengend Snippet: a Schematic representation of the establishment of luciferase constructs (Empty and h238 WT) and H4 stable cell lines. b, c Luciferase activity of H4 h238 WT treated with 100 µM NMDA, 100 µM L-glutamate (n=4, Dunnett’s test, p Ctrl vs NMDA =0.0038, p Ctrl vs L-Glu =0.0057) (b) , and 30 µM memantine (n=5, Student’s t -test, p=0.0003) (c) , respectively. d Schematic representation of luciferase constructs containing a deletion mutant and a motif mutant of the κB motif in the 238-bp KLK7 promoter region (h238 ΔκB motif and h238 mut-κB motif). e Luciferase activity of each luciferase construct transiently expressed in H4 cells (n=4, Dunnett’s test, p h238 WT vs h238 ΔκB motif =0.0432, p h238 WT vs h238 mut-κB motif =0.0318). f, g Luciferase activity of H4 h238 ΔκB motif (n=4, Dunnett’s test, p Ctrl vs NMDA =0.4847, p Ctrl vs L-Glu =0.3969) (f) and H4 h238 mut-κB motif (n=4, Dunnett’s test, p Ctrl vs NMDA =0.3716, p Ctrl vs L-Glu =0.5012) (g) treated with 100 µM NMDA or 100 µM L-glutamate. h, i Luciferase activity of H4 h238 ΔκB motif (n=5, Student’s t -test, p=0.8164) (h) and H4 h238 mut-κB motif (n=5, Student’s t -test, p=0.5011) (i) treated with 30 µM memantine. All data are shown as mean±SEM. *p<0.05, **p<0.01, ***p<0.001; n.s., non-significant.

    Article Snippet: For transient expression of the luciferase constructs in H4 cells, Lipofectamine ® LTX Reagent & PlusTM Reagent (Thermo Fisher Scientific, catalog #15338100) was used following the manufacturer’s protocol.

    Techniques: Luciferase, Construct, Stable Transfection, Activity Assay, Mutagenesis

    a Schematic representation of the canonical NF-κB activation pathway with the targets of the inhibitors IKK-16 and JSH-23 indicated. b The mRNA expression level of KLK7 in H4 cells treated with 2 µM IKK-16 or 30 µM JSH-23 (n=6, Student’s t -test, p Ctrl vs IKK-16 =0.0270, p Ctrl vs JSH-23 =0.0103). c The Aβ-degrading activity of H4 cells treated with 2 µM IKK-16 or 30 µM JSH-23. Representative immunoblots and quantified results are shown (Input=1.0, n=4, Student’s t -test, p Ctrl vs IKK-16 =0.0010, p Ctrl vs JSH-23 =0.0082). d Cell viability of H4 cells treated with 2 µM IKK-16 or 30 µM JSH-23 (n=4, Student’s t -test, p Ctrl vs IKK-16 =0.9618, p Ctrl vs JSH-23 =0.9043). e Luciferase activity of H4 h238 WT treated with 2 µM IKK-16 or 30 µM JSH-23 (n=4 (IKK-16), n=6 (JSH-23), Student’s t -test, p Ctrl vs IKK-16 <0.0001, p Ctrl vs JSH-23 <0.0001). All data are shown as mean±SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001; n.s., non-significant.

    Journal: bioRxiv

    Article Title: Inhibition of NF-κB signaling pathway in astrocytes facilitates amyloid-β clearance by kallikrein-related peptidase 7

    doi: 10.1101/2025.03.02.641088

    Figure Lengend Snippet: a Schematic representation of the canonical NF-κB activation pathway with the targets of the inhibitors IKK-16 and JSH-23 indicated. b The mRNA expression level of KLK7 in H4 cells treated with 2 µM IKK-16 or 30 µM JSH-23 (n=6, Student’s t -test, p Ctrl vs IKK-16 =0.0270, p Ctrl vs JSH-23 =0.0103). c The Aβ-degrading activity of H4 cells treated with 2 µM IKK-16 or 30 µM JSH-23. Representative immunoblots and quantified results are shown (Input=1.0, n=4, Student’s t -test, p Ctrl vs IKK-16 =0.0010, p Ctrl vs JSH-23 =0.0082). d Cell viability of H4 cells treated with 2 µM IKK-16 or 30 µM JSH-23 (n=4, Student’s t -test, p Ctrl vs IKK-16 =0.9618, p Ctrl vs JSH-23 =0.9043). e Luciferase activity of H4 h238 WT treated with 2 µM IKK-16 or 30 µM JSH-23 (n=4 (IKK-16), n=6 (JSH-23), Student’s t -test, p Ctrl vs IKK-16 <0.0001, p Ctrl vs JSH-23 <0.0001). All data are shown as mean±SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001; n.s., non-significant.

    Article Snippet: For transient expression of the luciferase constructs in H4 cells, Lipofectamine ® LTX Reagent & PlusTM Reagent (Thermo Fisher Scientific, catalog #15338100) was used following the manufacturer’s protocol.

    Techniques: Activation Assay, Expressing, Activity Assay, Western Blot, Luciferase